Journal: Cell reports

Article Title: Recursive feedback between matrix dissipation and chemo-mechanical signaling drives oscillatory growth of cancer cell invadopodia

doi: 10.1016/j.celrep.2021.109047

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 280 kDa Matrigel Corning Cat# 354230 Calcium Sulfate (CaSO4) Sigma Aldrich Cat# 255696-10G Lipofectamine 3000 ThermoFisher Scientific Cat# L3000075 OptiMEM ThermoFisher Scientific Cat# 31985062 EGF Peprotech, Inc. Cat# AF-100-15 Puromycin ThermoFisher Scientific Cat# A1113803 Neomycin ThermoFisher Scientific Cat# 21810031 Fluoresceinamine Sigma Aldrich Cat# 20162 Critical commercial assays MycoAlert Mycoplasma Detection Kit Lonza Cat# LT07-118 GeneJet Plasmid Miniprep kit ThermoFisher Scientific Cat# K0701 Deposited data Measured invadopodia dynamics of MDA-MB-231 This paper https://doi.org/10.17632/tp7fkkxk44.2 Measured circularity of HT-1080 cells Wisdom et al., 2018 https://doi.org/10.17632/tp7fkkxk44.2 Experimental models: Cell lines MDA-MB-231 ATCC ATCC® HTB-26 HT-1080 cells ATCC ATCC® CCL-121 Recombinant DNA RFP-LifeAct Gift from A. Dunn N/A Software and algorithms Simulation of invadopodia dynamics This paper N/A High throughput invadopodia dynamics analysis Chang et al., 2020 N/A MATLAB Mathworks https://www.mathworks.com/products/matlab.html Imaris Oxford Instruments https://imaris.oxinst.com/ ImageJ ImageJ https://imagej.net/Welcome Other Stress-controlled rheometer TA Instruments AR2000EX Open in a separate window KEY RESOURCES TABLE A chemo-mechanical model for invadopodia dynamics in 3D matrices is developed Oscillations occur when timescales for extension and myosin dynamics are comparable Matrix plastic strain accumulates by cyclic ratcheting during invadopodia growth High matrix plasticity facilitates the oscillatory growth of invadopodia

Techniques: Blocking Assay, Virus, Recombinant, Modification, Plasmid Preparation, Software, High Throughput Screening Assay

Journal: bioRxiv

Article Title: High speed functional imaging with a microfluidics-compatible open-top light-sheet microscope enabled by model predictive control of a tunable lens

doi: 10.1101/2025.07.23.666439

Figure Lengend Snippet: Open-top light-sheet microscope with electrically tunable lens (ETL) remote focusing under model predictive control. (a) Scan methods for volumetric light-sheet imaging. (i) In the simplest case, a sample is scanned through a static sheet. (ii) An actuated objective lens can follow a scanning light-sheet, which is faster than (i) but inertia limited and can cause problems with water immersion. (iii) Remote focusing optically shifts the focal plane using an active element like an ETL (b). This setup has less inertia and no moving parts at the sample. (c) Long-working distance open-top imaging is achieved using an asymmetric pair of objective lenses coupled with a water immersion fitting. This enables unobstructed imaging across a water-matched barrier such as FEP (above). (d) Maximum intensity projections along the Z (top) and Y (bottom) axes of a worm expressing a pan-neuronal nuclear-localized fluorescent protein positioned in a microfluidic channel as shown in (c). Scale bar 20 µ m. (e) Fast actuation of an ETL induces high frequency oscillation which slows response time. Performance is improved by using model predictive control to optimize drive signals. The controller iteratively optimizes the input signal to the ETL by minimizing simulated output error while obeying system constraints.

Article Snippet: Finally, we used the generated model to construct a model predictive controller using the MATLAB Model Predictive Control toolkit.

Techniques: Microscopy, Control, Imaging, Expressing